STEM-CELLBANKER® DMSO Free GMP grade is a chemically defined freezing solution that does not contain DMSO as an anti-freezing agent. It was developed for customers who do not prefer to use DMSO-containing cryopreservation solution due to a variety of their intended uses. It is completely free of serum and animal derived component, and contains only USP, EP, JP graded ingredients or JP excipients.
STEM-CELLBANKER® GMP grade is manufactured in a facility compliant with JP, EU, US and PIC/S GMP guidelines and the production and the quality are controlled in compliance with JP GMP guidelines.
STEM-CELLBANKER® GMP grade is manufactured in a facility compliant with JP, EU, US and PIC/S GMP guidelines and the production and the quality are controlled in compliance with JP GMP guidelines.
PRODUCT INTRODUCTION
Advantages:
- Free of serum and animal derived component.
- Ready-to-use with simple protocol.
- Save cost/time/steps with complete formulation, no further preparation, direct freezing at -80℃ or programmable freezing, etc.
- Safe cryopreservation with high quality ingredients, no risk of contamination animal-derived components, batch-to-batch stability.
- High quality product carefully monitored in compliance with GMP.
This product has been registered in MF within PMDA in 2016 and BBMF within CBER (Center for Biological Evaluation and Research)/FDA (the U.S. Food and Drug Administration) in 2019.
Store at 2 to 8 or below -20 degrees Celsius. In unopened condition and under storage temperatures (2-8°C ), the product will be stable for 3 years after the date of manufacture.
Specifications
Protocol:
A. Freezing
For optimum results, cells for cryopreservation should be in log phase of growth. Similar or standard freezing protocols may be substituted.
- Examine and make sure the cell culture free of contamination, in healthy situation and proper confluency, etc.
- 2Perform a cell count to determine the viability of cells.
- Gently pellet the cells centrifugation (3-5 minutes at 1,000~2,000rpm, 4℃). Remove the supernatant using a aspirator.
- Gently suspend the cells with STEM-CELLBANKER® (1ml for 5x105 - 5x106 cells).
- Dispense the cell suspension in 1ml aliquots to cryopreservation vials that have been labeled with the cell line name, cell concentration, passage date and other essential information.
- Place the vials directly in a -80℃ or in a programmable freezer for storage. If necessary, transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.
B. Thawing
- Remove the frozen cell from storage and quickly thaw in a 37°C shaking water bath.
- Immediately dilute and gently mix each 1ml of cells with 10ml of complete cell culture medium. The use of CELLOTION® is more effective.
- Gently pellet the cells centrifugation (3-5 minutes at 1,000~2,000rpm, 4℃). Remove the supernatant aspirator.
- Gently suspend the cells with appropriate volume of complete cell culture medium. And plate in a culture flask.
- Continue the further culture procedures according to standard protocols.
Application
When tested in the following cells with this product, 80-90% of viability of cells was observed.
| Cell type | Description |
| P3/x63-Ag8.U1 | Murine myeloma cell |
| 2D-8 | Murine hybridoma |
| YAC-1 | Murine lymphoblast |
| NBM-Lu | Normal newborn murine fibroblast cell line |
| 129SV | Mouse ES cell |
| Feline PBMC | Feline peripheral blood mononuclear cells |
| Canine PBMC | Canine peripheral blood mononuclear cells |
| Jurkat | Human T-cell line |
| SK007 | Human B-cell line |
| K562 | Human caucasian chronic myelogenous leukaemia cell |
| HeLa | HUman uterine cervical carcinoma cell |
| HepG2 | Human hepatocellular carcinoma cell |
| Caco-2 | Human colonic adenocarcinoma cell |
| UE6E7-16 | Human Mesenchymal cell |
| UE7T-13 | Human Mesenchymal Stem cell |
| 201B7 | Human iPS cell |
Order information
Package: 20ml, 100ml / bottle
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