PRODUCT INTRODUCTION
Phytohemagglutinin M (PHA-M) for Lymphocyte Stimulation is a mitogen used for the stimulation of cell proliferation in lymphocyte cell cultures. It is a lectin extracted from red kidney beans (Phaseolus vulgaris).
Phytohemagglutinin M consists of two molecular species, a leucoagglutinin (PHA-L) and an erythroagglutinin (PHA-E). Each of the proteins contains a family of five isolectins, each being a tetramer held together by non-covalent forces. PHA-M is the mucoprotein form.
Phytohemagglutinin M was originally used for separating leukocytes from whole blood. Its main application field is the stimulation of cell proliferation in lymphocyte cultures. It also has powerful erythroagglutinating properties.
Specifications
| Appearance | Frozen liquid |
| Storage and shelf life |
Store at ≤-15°C. Do not use this product after its expiry date. After thawing, the PHA-M is stable for at least 1 month at +2°C to +8°C. PHA-M may appear cloudy at +2°C to +8°C. The turbidity has no effect on the activity of PHA-M. |
| Shipping conditions | Frozen (Dry Ice) |
| Working concentration | 2 – 4 ml of PHA-M solution per 100 ml culture medium |
Application
Phytohemagglutinin is a lectin extracted from red kidney beans (Phaseolus vulgaris). The protein consists of two molecular species, a leucoagglutinin (PHA-L) and an erythroagglutinin (PHA-E). Each of the proteins contains a family of five isolectins, each being a tetramer held together by noncovalent forces. PHA-M is the mucoprotein form and is a crude extract that contains potent cell agglutinating and mitogenic activities and is most often utilized for the stimulation of cell division in lymphocyte cultures.
Application:
- Stimulates mitotic division of lymphocytes in cytogenetic and immunological applications
- Powerful erythroagglutinating properties
Phytohemagglutinin is a non-automatic, in vitro Diagnostic class A sterile product intended for laboratory use.
Order information
Phytohemagglutinin M (PHA-M)
Cat. No. PHA-H (10 ml)
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