Colcemid Solution (N-Deacetyl-N-methylcolchicine) is commonly used in chromosome analysis during lymphocyte karyotyping. It arrests cells in metaphase so the chromosomes can be separated for further investigation.
Colcemid is related to colchicine but is less toxic. Colcemid depolymerizes microtubules and limits their formation. Appropriate dilutions inactivate the spindle fiber formation, thus arresting cells in metaphase, and allowing cell harvest and karyotyping to be performed.
Appearance | Clear liquid |
CAS No. | 477-30-5 |
Storage and shelf life |
Store at +2°C to +8°C upon arrival. Do not use this product after its expiry date. Ok to freeze. Avoid repeated freeze-thaw cycles. Once opened store at +2°C to +8°C and use within 4-6 weeks. |
Shipping conditions | Ambient |
Working concentration | Recommended final concentration: 0.1 µg/ml |
Colcemid Solution (10 µg/ml) in DPBS
Cat. No. COL-H (10 ml)