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CELLBANKER® 1 is a ready-to-use cell cryopreservation medium with serum containing formulation for broad spectrum of mammalian cell cultures. The unique formulation allows for stable cryopreservation and high viability after freeze-thaw procedure, even for sensitive cell lines.


Advantages: Safe and Save!
Safe: high cell viability
Save: complete formulation, no further preparation, direct freezing at -80℃, no programmable freezer required, etc.

Comparative Analysis of Cellbanker® 1 vs. conventional cell cryopreservation method:

Storage and Stability: Store at 2 to 8 or below -20 degrees Celsius. In unopened condition and under storage temperatures (2-8℃), the product will be stable for 3 years after the date of manufacture.



A. Freezing
For optimum results, cells for cryopreservation should be in log phase of growth. Similar or standard freezing protocols may be substituted.
  1. Examine and make sure the cell culture free of contamination, in healthy situation and proper confluency, etc.
  2. Perform a cell count to determine the viability of cells.
  3. Gently pellet the cells centrifugation (3 - 5 minutes at 1,000~2,000rpm, 4℃). Remove the supernatant using a aspirator.
  4. Gently suspend the cells with CELLBANKER cryopreservation medium (1 ml for 5×105 - 5×106 cells).
  5. Dispense the cell suspension in 1ml aliquots to cryopreservation vials that have been labeled with the cell line name, cell concentration, passage date and other essential information.
  6. Place the vials directly in a -80℃ for storage. If necessary, transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.
IMPORTANT: Optimum protocol may change with the cell types.
B. Thawing
  1. Remove the frozen cell from storage and quickly thaw in a 37 ℃ shaking waterbath.
  2. Immediately dilute and gently mix each 1ml of cells with 10ml of complete cell culture medium.
  3. Gently pellet the cells centrifugation (3-5 minutes at 1,000 - 2,000rpm, 4℃). Remove the supernatant aspirator.
  4. Gently suspend the cells with appropriate volume of complete cell culture medium. And plate in a culture flask.
  5. Continue the further culture procedures according to standard protocols.


Cell type Description
293 Sheared human Ad5 DNA-transformed cell line.
293T Human cell line expressing SV40 large T antigen
32D Murine myeloid cell
3LL Murine Lewis lung cancer cell
A10 Thoracic aortic smooth muscle of an embryonic rat
A2781 Glioblastoma cell
Ac2F Rat liver cell
AtT20 Murine anterior pituitary cell
Ba/F3 Murine Pro-B cell
BHK-21 Ba Hamster renal fibroblast cell
C2C12 Mouse myoblast cell
C3H10T1/2 Murine embryonic fibroblast cell
Caco-2 Human colonic adenocarcinoma cell
CHL/1U Neonatal Chinese Hamster Lung cell
CHO Chinese Hamster Ovary cell
CHO-K1 Chinese Hamster Ovary cell
Colo203 Human colonic adenocarcinoma cell
COS1 African green monkey kidney cell
COS7 African green monkey kidney cell
CTLL-2 Murine T-cell
DLP-1 Human colonic adenocarcinoma cell
DT40 Chicken B lymphocyte cell
DU145 Human prostate carcinoma cell
EBV transformed B cell
EJ-1 Human bladder carcinoma
ELM-D Murine erythroblastic leukemia cell
HeLa cell Human Uterine Cervical Carcinoma Cell
Hep3B Human hepatoma cell
Hepal-6 Murine hepatoma cell
HepG2 Hepatocellular carcinoma cell
HL-60 Human promyelocytic leukemia cell
HT-2 Murine cell
Huh-7 Human hepatocarcinoma cell
Jurkat Human T-cell line
K-562 Human Caucasian Chronic myelogenous leukaemia cell
Kato-III Human stomach carcinoma
KM12-LX Human Caucasian Chronic myelogenous leukaemia cell
L5178Y Murine lymphoma cell
L929 Murine fibroblast cell
LM Murine fibroblast cell
LNCap Human prostate adenocarcinoma cell
MCF-7 Human metastatic mammary carcinoma cell
MDCK Madin-Dar canine kidney cell
Microvascular Endothelial Cell
Molt-4 Human T-cell
NB-1/GOTO Human neuroblastoma
NCI-H441 Human lung adenocarcinoma epithelial cell
NIH3T3 Mouse embryonic fibroblast cell
P388 Murine leukimia cell
P3/x63-Ag8.U1 Murine myeloma cell
PC12 Rat pheochromocytoma cell
Periodontal ligament fibroblast cell
Periodontal ligament membrane fibroblast cell
Raji Human B-cell line
RAW264.7 Murine Macrophage cell
sf9 Insect cell
SK-N-MC Neuroepithelioma cell
SN12C Renal carcinoma cell
Subclone: PM6,Clone8, MM3
Stem cell  
Stomach carcinoma cell
THle3 Human liver cell
T24 Human bladder carcinoma
Vero African green monkey kidney epithelial cell
WEHI3B Murine myeloid leukemia
WiDr Human colon adenocarcinoma cell

Typical experimental results

Cells Preservation period
Viability of cells (%)
-80℃ -196℃
Hybridoma 10 95 95
Myeloma 10 90 90
L929 10 90 90
FM3A 5 90 90
BALB/3T3 5 90 90
M1 5 90 90
YAC-1 5 90 -
RLC-16 5 90 90
NRK 5 90 90
PC-12 5 90 -
CHO 5 90 90
V79 5 90 90
COS-1 5 90 90
Vero 5 90 90
Kidney-derived tumor cell 5 90 90
EBV transformed cell 5 90 90
HEL-derived fibroblast 5 90 90
Melanoma 5 90 90
Caco-2 3 90 -
C-5 5 90 90
CEM 5 90 90
K562 10 90 90
Jurkat 10 90 90
BALL-1 5 90 90
HUC-Fm 5 80 80


Order information

Package: 20 ml, 100 ml/bottle




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