Catalog number: TRY-3B (100 ml)
Trypsin-EDTA solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.
PRODUCT INTRODUCTION
Trypsin-EDTA solutions are used for the detachment of adherent cells from culture surfaces. They are composed of natural porcine pancreas-derived trypsin and EDTA.
The addition of phenol red serves as a pH indicator to adjust to the perfect cell culture conditions.
The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.
Specifications
| Appearance | Clear frozen liquid |
| Storage and shelf life |
Store at ≤-15°C. Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended. Once opened, store at 4°C and use within 2-4 weeks. |
| Shipping conditions | Frozen (Dry ice) |
| Thawing | +37°C water bath or overnight at +2°C to +8°C. Swirl gently to homogenize. |
Application
Trypsin-EDTA solutions are used to detach adherent cells from culture surfaces. They are composed of natural porcine pancreas-derived trypsin and EDTA. The addition of phenol red serves as pH indicator to adjust to the perfect cell culture conditions. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.
Order information
Trypsin-EDTA (0.25 %) in HBSS (1x) with Phenol Red
Cat. No. TRY-3B (100 ml)
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