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Catalog number: TRY-4B (100 ml)
Trypsin-EDTA solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.
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PRODUCT INTRODUCTION

Trypsin-EDTA solutions are used for the detachment of adherent cells from culture surfaces. They are composed of natural porcine pancreas-derived trypsin and EDTA.

The addition of phenol red serves as a pH indicator to adjust to the perfect cell culture conditions.

The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.

Specifications

Appearance Clear frozen liquid
Storage and shelf life

Store at ≤-15°C.

Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended.

Once opened, store at 4°C and use within 2-4 weeks.

Shipping conditions Frozen (Dry ice)
Thawing +37°C water bath or overnight at +2°C to +8°C. Swirl gently to homogenize.

 

Application

Trypsin-EDTA solutions are used to detach adherent cells from culture surfaces. They are composed of natural porcine pancreas-derived trypsin and EDTA. The addition of phenol red serves as pH indicator to adjust to the perfect cell culture conditions. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.

Order information

Trypsin-EDTA (0.05 %) in HBSS (1x) with Phenol Red

Cat. No. TRY-4B (100 ml)

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