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Stable Glutamine (also known as L-Alanyl-L-Glutamine Solution) is a direct substitute for L-Glutamine. This amino acid with increased stability is usually supplemented to cell culture media.
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PRODUCT INTRODUCTION

Stable Glutamine (also known as L-Alanyl-L-Glutamine Solution) is a direct substitute for L-Glutamine. L-Glutamine is an essential amino acid that is a crucial component of culture media and serves as a major energy source for cells in culture.

L-Glutamine in liquid media degrades relatively rapidly when cultured at +37 °C and toxic degradation products (ammonium ions) can interfere and cause damage to cells.

Stable Glutamine is a stable dipeptide form of L-Glutamine. Unlike L-Glutamine, Stable Glutamine does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-Glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-Glutamine during long-term cultures.

This product, L-Alanyl-L-Glutamine Solution, is prepared in cell culture-grade water and comes at a concentration of 200 mM.

Specifications

Appearance Clear frozen liquid
Storage and shelf life

Store at ≤-15°C.

Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended.

Once opened, store at +4°C and use within 4-6 weeks.

Shipping conditions Frozen (Dry ice)
Thawing +37°C water bath or overnight at +2°C to +8°C. Swirl gently to homogenize.

 

Application

L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine in liquid media degrades relatively rapidly when cultured at +37°C and toxic degradation products (ammonium ions) can interfere and may cause damage to cells. Stable Glutamine is a stable dipeptide form of L-Glutamine. Unlike L-Glutamine, Stable Glutamine does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-Glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-Glutamine during long-term culture. This solution is prepared in cell culture grade water.

Applications:

  • Direct substitute for L-Glutamine
  • For mammalian cell cultures
  • For suspension and adherent cell cultures
  • Essential media component

Order information

Appearance Clear frozen liquid
Storage and shelf life

Store at ≤-15°C.

Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended.

Once opened, store at +4°C and use within 4-6 weeks.

Shipping conditions Frozen (Dry ice)
Thawing +37°C water bath or overnight at +2°C to +8°C. Swirl gently to homogenize.

 

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