PRODUCT INTRODUCTION
Penicillin/Streptomycin (Pen/Strep) is a ready-to-use antibiotic mix of penicillin and streptomycin. It is widely used in cell culture and can be added to cell culture media.
Penicillin/Streptomycin is effective against contaminations with gram-positive and gram-negative bacteria. This product is provided as a 100x concentrate.
Penicillins belong to the β-lactam family and act by inhibiting an essential enzyme for the synthesis of new membrane components (D-Alanin-Transpeptidase). Therefore penicillin is only effective on actively dividing cells.
Streptomycin, on the other hand, acts by binding to the 30S subunit of the bacterial ribosome. This mechanism inhibits protein synthesis within the bacterium, regardless of whether the cell is actively dividing or not.
Specifications
| Appearance | Clear frozen liquid |
| Storage and shelf life |
Store at ≤-15°C. Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended. Once opened, store at +4°C and use within 4-6 weeks. |
| Shipping conditions | Frozen (Dry ice) |
| Thawing | Overnight at +2°C to +8°C. Swirl gently to homogenize. |
| Working concentration | Recommended final concentration: 10 ml/L |
Application
The antibiotics penicillin and streptomycin are used in cell culture to prevent bacterial contamination due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin acts by interfering with the turnover of the bacterial cell wall. Streptomycin acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria. Penicillin/Streptomycin is provided as a ready to use 100x concentrate.
Applications:
- Prevention of cell culture contamination
- Most common antibiotic solution for the culture of mammalian cells
Order information
Penicillin/Streptomycin (100x)
Cat. No. PS-B (100 ml)
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