PRODUCT INTRODUCTION
Penicillin/Streptomycin (Pen/Strep) is a ready-to-use antibiotic mix of penicillin and streptomycin. It is widely used in cell culture and can be added to cell culture media.
Penicillin/Streptomycin is effective against contaminations with gram-positive and gram-negative bacteria. This product is provided as a 100x concentrate and is supplemented with L-Glutamine.
With a convenient one-step supplementation Penicillin/Streptomycin with L-Glutamine helps researchers to save time while reducing risks for contamination caused by multiple invasive supplementation schedules.
Penicillins belong to the β-lactam family and act by inhibiting an essential enzyme for the synthesis of new membrane components (D-Alanin-Transpeptidase). Therefore penicillin is only effective on actively dividing cells.
Streptomycin, on the other hand, acts by binding to the 30S subunit of the bacterial ribosome. This mechanism inhibits protein synthesis within the bacterium, regardless of whether the cell is actively dividing or not.
L-Glutamine is an essential amino acid and a crucial component of culture media. It serves as a major energy source for cells in culture.
Specifications
| Appearance | Clear frozen liquid |
| Storage and shelf life |
Store at ≤-15°C. Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended. Once opened, store at +4°C and use within 4-6 weeks. |
| Shipping conditions | Frozen (Dry ice) |
| Thawing | Overnight at +2°C to +8°C. Swirl gently to homogenize. |
| Working concentration | Recommended final concentration: 10 ml/L |
Application
With a convenient one-step supplementation Penicillin/Streptomycin with L-Glutamine helps researchers to save time while reducing risks for contamination caused by multiple invasive supplementation schedules. The antibiotics penicillin and streptomycin are used in cell culture to prevent bacterial contamination due to their effective combined action against grampositive and gram-negative bacteria. Penicillin acts by interfering with the turnover of the bacterial cell wall. Streptomycin acts by binding to the 30S subunit ofthe bacterialribosome, leading to inhibition of protein synthesis and death in susceptible bacteria. L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. Penicillin/Streptomycin with L-Glutamine is provided as a ready to use 100x concentrate.
Applications:
- Convenient one-step supplementation
- Reduces contamination risks
Order information
Penicillin/Streptomycin (100x), with L-Glutamine
Cat. No. PSG-B (100 ml)
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