PRODUCT INTRODUCTION
NCS21 Neuronal Supplement is a serum-free supplement for neuronal cell cultures. It is an optimized and modified formulation of B27® Supplement (B27® is a registered trademark of Life Technologies Corporation).
NCS21 Neuronal Supplement is suitable for the viable, long-term growth of hippocampal neurons and other neurons of the central nervous system (CNS) and peripheral nervous system (PNS). It is chemically defined and contains vitamins, hormones, and other growth factors, including insulin, human transferrin, catalase, antioxidants, and fatty acids.
Suitable applications include, e.g., differentiation of ES cells into neuron lineage (neuron and astrocytes), differentiation of neuronal stem cells into astrocytes and neurons, optimal growth and long-term survival of rat hippocampal neurons (fetal and adult), survival of neurons from embryonic rat striatum, substantia nigra, septum and cortex, and neonatal rat cerebellum (fetal and adult).
Specifications
| Appearance | Clear frozen liquid |
| Storage and shelf life |
Store at ≤-15°C. Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended. Once opened, store at 4° C and use within 2-4 weeks. |
| Shipping conditions | Frozen (Dry ice) |
| Thawing | +37°C water bath or overnight at +2°C to +8°C. Swirl gently to homogenize. |
| Working Concentrations | Recommended final concentration: 1 x |
Application
NCS21 Supplement is a serum-free supplement for neuronal cell cultures. It is an optimized and modified formulation of B27® Supplement (B27® is a registered trademark of Life Technologies Corporation). NCS21 Supplementis suitable forthe long-term growth and viability of hippocampal and other neurons ofthe central nervous (CNS) and peripheral nervous system (PNS). It is chemically defined and contains vitamins, hormones and other growth factors including insulin, human transferrin, catalase, antioxidants and fatty acids.
Applications:
- Differentiation of ES cells into neuron lineage (neuron and astrocytes)
- Differentiation of neuronal stem cells into astrocytes and neurons
- Optimal growth and long-term survival of rat hippocampal neurons (fetal and adult)
- Survival of neurons from embryonic rat striatum, substantia nigra, septum and cortex, and neonatal rat cerebellum (fetal and adult)
Order information
NCS21 Supplement (50x), Serum-free
Cat. No. C21-H (10 ml)
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