PRODUCT INTRODUCTION
HEPES is a buffer solution (1 M), also known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid. HEPES is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It maintains the pH in a range of 6.7 – 8.6 and the addition of 10 to 25 mM HEPES provides extra buffering capacity when a cell culture requires extended periods of manipulation outside of a CO2 incubator.
Our HEPES buffer solution is suitable for many cell culture systems because it is membrane impermeable, has a limited effect on biochemical reactions, and is chemically and enzymatically stable. The solution is prepared in cell culture grade water.
Specifications
| Appearance | Clear liquid |
| CAS No. | 7365-45-9 |
| Storage and shelf life |
Store at +2°C to +8°C protected from light. Once opened store at +2°C to +8°C and use within 24 months. |
| Shipping conditions | Ambient |
| Working concentration | Recommended final concentration: 10 – 40 mM |
Application
Order information
HEPES Buffer Solution, 1 M
Cat. No. HEP-B (100 ml)
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