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Catalog number: HEP-B (100 ml)
HEPES Buffer Solution (1M) is a biological buffer used in cell culture media. Due to its stability, it is suitable for many different cell culture systems.
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PRODUCT INTRODUCTION

HEPES is a buffer solution (1 M), also known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid. HEPES is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It maintains the pH in a range of 6.7 – 8.6 and the addition of 10 to 25 mM HEPES provides extra buffering capacity when a cell culture requires extended periods of manipulation outside of a CO2 incubator.

Our HEPES buffer solution is suitable for many cell culture systems because it is membrane impermeable, has a limited effect on biochemical reactions, and is chemically and enzymatically stable. The solution is prepared in cell culture grade water.

Specifications

Appearance Clear liquid
CAS No. 7365-45-9
Storage and shelf life

Store at +2°C to +8°C protected from light.

Once opened store at +2°C to +8°C and use within 24 months.

Shipping conditions Ambient
Working concentration Recommended final concentration: 10 – 40 mM

 

Application

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It maintains the pH in a range of 6.7 – 8.6 and the addition of 10 to 25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator. HEPES is suitable for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, and is chemically and enzymatically stable. HEPES Buffer Solution is prepared in cell culture grade water

Order information

HEPES Buffer Solution, 1 M

Cat. No. HEP-B (100 ml)

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