G-418 Sulfate Solution, 100 % Activity (50 mg/ml)

Name: G-418 Sulfate Solution, 100 % Activity (50 mg/ml)
Brand: Capricorn Scientific
SKU: 202401-0241
Rating: 4.9 (45 reviews)

G-418 Sulfate Solution is used as a selection antibiotic in cell culture. It blocks polypeptide synthesis. Transformed cells containing the neomycin resistance genes neo can be selected.

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G-418 Sulfate Solution, 100 % Activity (50 mg/ml)

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PRODUCT INTRODUCTION
Specifications
Application
Order information
Customer reviews

PRODUCT INTRODUCTION

G-418 Sulfate Solution is used in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.

G-418 Sulfate is an aminoglycoside antibiotic, related to Gentamicin, and exhibits toxicity towards both eukaryotic and prokaryotic cells. It is produced by Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells.

Specifications

Appearance	Clear frozen liquid
CAS No	108321-42-2
Storage and shelf life	Store at ≤ -15°C. Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended. Once opened, store at +4°C and use within 4-6 weeks.
Shipping conditions	Frozen (Dry Ice)
Thawing	Overnight at +2°C to +8°C. Swirl gently to homogenize.
Working concentration	Recommended final concentration (0.1 – 1.0 mg/ml) depending on the cell type: HeLa: 200 – 600 µg/ml 3T3 cells: 500 – 1000 µg/ml CHO: 200 – 400 µg/ml HEK 293: 500 – 800 µg/ml Jurkat cells: 600 – 700 µg/ml

Application

G-418 is used in the selection and maintenance of eukaryotic cells, stable transfected with neomycin resistance genes. G-418 is an aminoglycoside antibiotic, related to Gentamicin, and exhibits toxicity towards both eukaryotic and prokaryotic cells. It is produced by Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells.