G-418 Sulfate Powder is used as a selection antibiotic in cell culture. It blocks polypeptide synthesis. Transformed cells containing the neomycin resistance genes neo can be selected.
PRODUCT INTRODUCTION
G-418 Sulfate Powder is used in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.
G-418 Sulfate is an aminoglycoside antibiotic, related to Gentamicin, and exhibits toxicity towards both eukaryotic and prokaryotic cells. It is produced by Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells.
Specifications
| Appearance | White to off-white powder |
| CAS No. | 108321-42-2 |
| Storage and shelf life | Store at +2°C to +8°C. Please refer to product label for expiration date. |
| Shipping conditions | Ambient |
| Working concentration |
Recommended final concentration (0.1 – 1.0 mg/ml) depending on the cell type:
|
Application
G-418 is used in the selection and maintenance of eukaryotic cells, stable transfected with neomycin resistance genes. G-418 is an aminoglycoside antibiotic, related to Gentamicin, and exhibits toxicity towards both eukaryotic and prokaryotic cells. It is produced by Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells
Order information
G-418 Sulfate Powder
Cat. No. G418-Q (5 g), G418-R (10 g)
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