Catalog number: EC64405
Bioendo EC Endotoxin Test Kits provide a kind of measurement for quantitative endotoxin testing solution. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored product with maximum absorbance at 405 nm. For End-point Chromogenic Endotoxin Test Kit without Diazo Coupling, a regular spectrophotometer or microplate reader is required to read the absorbance at 405nm. The regular spectrophotometer with the function of kinetic experiment, the accuracy of temperature control and 405nm filter, then use that spectrophotometer can read the quantitative endotoxin result of EC kit.
PRODUCT INTRODUCTION
End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is conducted by adding a colorless artificial peptide substrate solution into mixture of Lyophilized Amebocyte Lysate and test sample after a certain incubation period. If test sample contain endotoxin, a yellow color will develop in the 96 well microplate. Its absorbance (λmax = 405 nm) is related to the endotoxin concentration. Endotoxin concentration of test sample could be calculated based on a standard curve.
Specifications
Sensitivity Range: 0.01 - 0.1 EU/ml, matching assay time is about 46 minutes
0.1 - 1 EU/ml, matching assay time is about 16 minutes.
Application
End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into the mixture of Lyophilized Amebocyte Lysate and test sample after a certain incubation period. Yellow color develops if test sample contains endotoxin. Then use regular spectrophotometer or plate reader to read the absorbance at 405 nm. End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is suitable for all kinds of biomedical samples such as protein, vaccine, plasmid, DNA, RNA endotoxin assay.
Order information
| Catalog No. | Description | Kit Contents |
Sensitivity (EU/ml) |
| EC64405 |
Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay), 64 Tests/Kit |
2 Lyophilized Amebocyte Lysate, 1.7 ml/vial; 2 Water for BET, 50 ml/vial; 2 CSE; 4 Chromogenic Substrate, 1.7 ml/vial; |
0.1 – 1 EU/ml |
| EC64405S |
Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay), 64 Tests/Kit |
2 Lyophilized Amebocyte Lysate, 1.7 ml/vial; 2 Water for BET, 50 ml/vial; 2 CSE; 4 Chromogenic Substrate, 1.7 ml/vial; |
0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
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