PRODUCT INTRODUCTION
Why choose CELLOTION® Cell wash and recovery solution?
A row of cell washes for each cell isolation or post-thawing manipulation may cause plenty of cell loss, particularly if the process conducted only with serum and protein-free medium. Imagine that as much as 40% of your valuable cells may go down into the drain unawarely every time after cell wash, CELLOTION® ensures you with better yields of cells.
Figure 1. Comparative Analysis of CELLOTION Cell Wash and Recovery Solution vs. General-use Cell Wash Technologies
Figure 2. Comparative Analysis (multiple washing procedures) of CELLOTION Cell Wash and Recovery Solution vs. General-use Cell Wash Technologies
- Advantages:
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- Significantly increased cell yield after washing procedures while maintaining cell viability.
- Chemically defined solution with serum and animal derived component-free formulation.
- Simply protocol.
Store at 2 to 8 degrees Celsius. In unopened condition and under storage temperatures, the product will be stable for 3 years after the date of manufacture.
Specifications
- Protocol: Use CELLOTION® as a substitution for general-use cell wash solution such as PBS buffer, saline or culture medium with standards cell wash procedures.
- Example of use: Monocytes isolation with CELLOTION® Cell Wash and Recovery Solution.
- Gently dilute the peripheral blood or umbilical cord blood (UCB) with CELLOTION® Cell Wash and Recovery Solution 1:1. Gently add Ficoll-hypaque solution to the mixture 1:2. Do not mix and the blood should remain on top.) Centrifuge the tube for 30minutes at 1,800rpm, at room temperature. Collect the buffy coat (white blood cells ring) carefully with a sterile pipette tip without touching the Ficoll-hypaque solution. Gently dilute the cells with CELLOTION®, then wash for 2 - 3 times in CELLOTION®. Count the number of cells.
Application
- Stem cell isolation from a variety of sources
- Leukocyte isolation from peripheral blood
- Post-thawing cell wash
- General cell wash
Order information
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SDS : CELLOTION®
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