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Antibiotic/Antimycotic Solution can be added to cell culture media. It prevents bacterial and fungal contaminations of cell cultures. This solution contains penicillin, streptomycin, and amphotericin B.


Our Antibiotic/Antimycotic Solution is used to prevent bacterial and fungal contamination in cell culture applications. It contains a combination of penicillin and streptomycin, acting against gram-positive and gram-negative bacteria, and amphotericin B, which acts against yeast and multicellular fungi.

The Antibiotic/Antimycotic Solution is provided as a ready-to-use 100x concentrate - no solubilization necessary. This product is viable for tissue culture, bacteria media, and many other applications.

Penicillins belong to the β-lactam family and act by inhibiting an essential enzyme for the synthesis of new membrane components (D-Alanin-Transpeptidase). Therefore penicillin is only effective on actively dividing cells.

Streptomycin, on the other hand, acts by binding to the 30S subunit of the bacterial ribosome. This mechanism inhibits protein synthesis within the bacterium, regardless of whether the cell is actively dividing or not.

Amphotericin B is a fungizide that binds with ergosterol in the fungus membrane, leading to formation of pores. By this process, the fungus starts leaking important ions, finally resulting in cell death.


Appearance Clear yellowish frozen liquid
Storage and shelf life

Store at ≤-15°C.

Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended.

Once opened, store at +4°C and use within 4-6 weeks.

Shipping conditions Frozen (Dry ice)
Thawing Overnight at +2°C to +8°C. Swirl gently to homogenize.
Working Concentrations Recommended final concentration: 10 ml/L



Antibiotic/Antimycotic Solution is used to prevent bacterial and fungal contamination. It contains a combination of penicillin and streptomycin acting against gram-positive and gram-negative bacteria, and amphotericin B, which acts against yeast and multicellular fungi. Antibiotic/Antimycotic Solution is provided as a ready to use 100x concentrate.


  • Broad range antibiotic (penicillin and streptomycin)
  • Antifungal spectrum (amphotericin B)
  • Suitable for contaminated cells
  • Recommended as primary cell line prophylaxis

Order information

Antibiotic/Antimycotic Solution (100x)

Cat. No. AAS-B (100 ml)

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