PRODUCT INTRODUCTION
Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes. It has been developed to meet the most demanding requirements for gentle and effective detachment of adherent cells. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion-coated plasticware.
The advantages of Accutase over the traditional Trypsin/EDTA treatment are that it is less damaging to cells, thus leading to increased viability, and no inactivation of the enzymes by inhibitors or serum is required.
Specifications
| Appearance | Clear, pale red to orange solution |
| Storage and shelf life | Store at ≤-15°C. Once defrosted, store at +2°C to +8°C. Note: Do not thaw the product in a 37°C water bath. After thawing, the Accutase solution may be stored for up to 2 months at +2°C to +8°C. Do not store at room temperature. |
| Shipping conditions | Frozen (Dry ice) |
| Working concentration | 1x (ready-to-use) |
| Origin | Crustacea |
Application
Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes. It has been developed to meet the most demanding requirements for gentle and effective detachment of adherent cells. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. The advantages of Accutase over the traditional Trypsin/EDTA treatment are that it is less damaging to cells leading to increased viability and no inactivation of the enzymes by inhibitors or serum is required. The Accutase solution does not contain mammalian or bacterial-derived products.
Applications:
- Gentle detachment of a vast variety of cells including very sensitive cell lines e.g. hESCs
- Routine cell passage
- Analysis of cell surface markers
- Quiescence assays by serum starvation
- Transformation assays by oncogene transfection
- Neural crest cell migration assays, tumor cell migration assays
- Cell proliferation, apoptosis
- Production scale-up (bioreactor)
- Flow cytometry
Order information
Accutase Cell Detachment Solution
Cat. No. ACC-1B (100 ml)
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