PRODUCT INTRODUCTION
RPMI 1640 (RPMI) is a growth medium used in different cell culture applications. RPMI stands for Roswell Park Memorial Institute, where it was developed in 1966.
This formulation of RPMI powder medium is supplemented with L-Glutamine & with 25 mM HEPES and comes without Sodium Bicarbonate (NaHCO3).
RPMI was developed to support lymphoblastoid cells in suspension cultures. It can also support a wide range of other cell types such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Originally intended to be supplemented with serum, RPMI 1640 has been shown to also support several cell lines in the absence of serum.
Powder media can be reconstituted to provide basic nutrients for all cell culture applications. We offer dry powdered cell culture media and buffers which are manufactured using the highest quality ingredients. The powder is precisely blended in clean, controlled state-of-the-art equipment to eliminate contaminating dust and to control temperature and humidity.
Specifications
| Appearance | Off-white to creamy white, homogenous powder |
| pH w/o Sodium Bicarbonate | 6.40 – 7.00 |
| pH with Sodium Bicarbonate | 6.70 – 7.30 |
| CO2 concentration optimum for liquid medium | 4.5 % |
| Storage and shelf life |
Store at +2°C to +8°C, dry and protected from light. Please refer to product label for expiration date. |
| Shipping conditions | Ambient |
| Use at | 16.4 g/L |
| Add | 2.0 g/L Sodium Bicarbonate |
Application
Order information
RPMI 1640 Powder Medium with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate
Cat. No. RPMI-HA-P10 (10L), RPMI-HA-P50 (50L)
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