Bioendo rCR Endotoxin Test Kit (Recombinant Cascade Reagents Chromogenic Assay) uses gene recombinant technology to express Factor C pathway serine zymogen proteases of horseshoe crab amebocyte in eukaryotic cells.
PRODUCT INTRODUCTION
The cascade reagents include Factor C which can be activated by bacterial endotoxins, Factor B and proclotting enzyme. Endotoxins bind to and convert Factor C to its active form, which subsequently activates Factor B, which in turn activates the proclotting enzyme. The activated proclotting enzyme then cleaves chromogenic substrate, releasing a yellow-colored pNA. The released pNA can be measured photometrically at 405 nm. Based on which, the endotoxin concentration of test sample can be quantified. rCR endotoxin test kit does not use animal source materials, protects the horseshoe crabs.
Specifications
Features:
1.Convenient detection: the same detection instrument as the Chromogenic LAL reagent is used, and there is no need to replace the detection instrument.
2.High sensitivity: the sensitivity can be as high as 0.001 EU/ml.
3.Stability: good repeatability between production batches
4.Specificity: does not react with fungal (1,3)-β-D-glucan, avoiding false positives caused by factor G bypass.
Application
Order information
| Catalog number | Specification | Detection range |
| RCR0428S |
4 Recombinant Cascade Reagents — 2.8 ml/vial 4 Reconstitution Buffer — 3.0 ml/vial 2 Control Standard Endotoxin — CSE10V 2 Water for BET — 50 ml/vial |
0.001 - 10 EU/ml |
| RCR0428 |
4 Recombinant Cascade Reagents — 2.8 ml/vial 4 Reconstitution Buffer — 3.0 ml/vial 2 Control Standard Endotoxin — CSE10V 2 Water for BET — 50 ml/vial |
0.005 - 5 EU/ml |
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