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Insulin-Transferrin-Selenium (ITS) is a supplement for basal cell culture media. Supplementation to media reduces the FBS requirements for routine cultivation of many cell lines.
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PRODUCT INTRODUCTION

Insulin-Transferrin-Selenium (ITS) is a chemically-defined supplement for a variety of cell culture applications. Most commonly, Insulin-Transferrin-Selenium (ITS) Solution is used as a supplement for basal cell culture media.

Our ITS supplement contains components that are required for the optimal performance of serum-free media. Supplementation of ITS to different conventional nutrient media substantially reduces the FBS requirement for routine maintenance of many cell lines.

Insulin-Transferrin-Selenium (ITS) is provided as a 100x concentrate in Earle's Balanced Salt Solution without Phenol Red.

Specifications

Appearance Clear solution
Storage and shelf life Store at +2°C to +8°C protected from light. Once opened, store at 4° C and use within 2-4 weeks.
Shipping conditions Ambient
Working Concentrations Recommended final concentration: 10 ml/L

Application

ITS (Insulin, Transferrin, Selenium) is a chemically defined supplementfor a variety of cell culture applications. ITS supplement contains components which are required for optimal performance of serum-free media. Supplementation of ITS to different conventional nutrient media substantially reduces the FBS requirement for routine maintenance of many cell lines. ITS is provided as a 100x concentrate.

Applications:

  • Serum-free cultivation
  • Reduction of FBS requirements
  • Cultivation of e.g. CHO, Hybridoma, MDCK and Vero cells
  • Supplement in murine embryonic stem cell culture

Order information

ITS (100x), Insulin, Transferrin, Selenium

Cat. No. ITS-H (10 ml)

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