PRODUCT INTRODUCTION
Hybridoma Supplement is a chemically-defined supplement for cell culture media that supports the growth of hybridoma cells. It contains insulin, ethanolamine, hydrocortisone, retinoic acid, linoleic acid, and other growth-promoting factors.
As an additive to culture medium, Hybridoma Supplement supports the growth of hybridoma in a manner feeder cells have been used before. The disadvantages occurring by the use of feeder cells may include overgrowth of newly formed hybridomas, source of contamination, competition for nutrients, and variations in growth factor concentrations.
Hybridoma Supplement can be used in culture medium under serum-free conditions. The low-protein content facilitates the isolation and purification of produced antibodies. Hybridoma Supplement is provided as a 10x concentrate.
Specifications
| Appearance | Clear red orange solution |
| Storage | Store at ≤-15°C. |
| Shipping conditions | Frozen (dry ice) |
| Working concentration | Recommended working concentration: 5 % to 10 % |
Application
Hybridoma cells result from fusion of an antibody producing B-cell from the immune system with a tumor cell. To support hybridoma development and achieve an optimal cell density, as well as cloning efficiency, growth factors and serum (10 % to 20 %) are required. Hybridoma Supplement is a chemically defined growth promoting supplement, containing insulin, ethanolamine, hydrocortisone, retinoic acid, linoleic acid, and other growth promoting factors. As supplement to culture medium it supports the growth of hybridoma in a manner feeder cells have been used before. The disadvantages occurring by the use of feeder cells may include: overgrowth of newly formed hybridomas, source of contamination, competition for nutrients, and variations in growth factor concentrations. Hybridoma Supplement can be used in culture medium under serum-free conditions. The low protein content facilitates the isolation and purification of produced antibodies. Hybridoma Supplement is provided as a 10x concentrate.
Applications:
- Improvement of Hybridoma Growth after fusion.
- Improvement of cloning efficiency of hybridomas
- Production of Monoclonal Antibodies under serum-free conditions.
Order information
Hybridoma Supplement
Cat. No. HCS-F (50 ml)
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