HEK|ONE T is a serum-free, chemically-defined, animal-component-free medium for transient HEK293 cells. It was developed for the cultivation and expression of HEK293 and other mammalian cell lines, with a special focus on transfection applications and virus production.
Our HEK|ONE T medium comes without L-Glutamine and is supplemented with Growth Hormones.
The medium is highly suitable for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI).
HEK|ONE T supports cell growth and production of recombinant proteins and antibodies in suspension culture. It can be used in research and further manufacturing.
Appearance | Clear yellow orange solution |
Formulation | w/o Anti-Clumping Agents |
Glucose Concentration | 7.0 g/L |
Glutamine | No glutamine; supplement with 6 – 8 mM L-glutamine prior to use |
Storage and Shelf Life | +2°C to +8°C; protected from light. Please refer to the label for expiry date. |
Shipping Conditions | Ambient |
Specifications |
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HEK|ONE T Chemically Defined Medium for Transient Expression, w/o L-Glutamine, with Growth Hormones, sterile-filtered
Cat. No. HEKT-1000ML