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CELLBANKER® 2 is a serum-free cell cryopreservation medium with unique complete formulation for broad spectrum of mammalian cell cultures. The unique formulation allows for stable cryopreservation and high viability after freeze-thaw procedure, without potential risk of contamination serum.

Optimum for serum-free cultured cells and peptide/protein expressing cells cryopreservation.

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PRODUCT INTRODUCTION

CELLBANKER® 2 is a serum-free cell cryopreservation medium with unique complete formulation for broad spectrum of mammalian cell cultures. The unique formulation allows for stable cryopreservation and high viability after freeze-thaw procedure, without potential risk of contamination serum.

Optimum for serum-free cultured cells and peptide/protein expressing cells cryopreservation.

Advantages: Safe and Save!

  • Safe: high cell viability, no risk of contamination serum derived pathogens, batch-to-batch stability.
  • Save: complete formulation, no further preparation, direct freezing at -80℃, no programmable freezer required, etc.

Store at 2 to 8 or below -20 degrees Celsius.In unopened condition and under storage temperatures (2-8℃), the product will be stable for 3 years after the date of manufacture.

Specifications

A. Freezing

For optimum results, cells for cryopreservation should be in log phase of growth. Similar or standard freezing protocols may be substituted.

  1. Examine and make sure the cell culture free of contamination, in healthy situation and proper confluency, etc.
  2. Perform a cell count to determine the viability of cells.
  3. Gently pellet the cells centrifugation (3 - 5 minutes at 1,000~2,000rpm, 4℃). Remove the supernatant using a aspirator.
  4. Gently suspend the cells with CELLBANKER® cryopreservation medium (1 ml for 5x105 - 5x106 cells).
  5. Dispense the cell suspension in 1ml aliquots to cryopreservation vials that have been labeled with the cell line name, cell concentration, passage date and other essential information.
  6. Place the vials directly in a -80℃ for storage. If necessary, transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.

IMPORTANT: Optimum protocol may change with the cell types.

B. Thawing

  1. Remove the frozen cell from storage and quickly thaw in a 37℃ shaking waterbath.
  2. Immediately dilute and gently mix each 1ml of cells with 10ml of complete cell culture medium.
  3. Gently pellet the cells centrifugation (3-5 minutes at 1,000 - 2,000rpm, 4℃). Remove the supernatant aspirator.
  4. Gently suspend the cells with appropriate volume of complete cell culture medium. And plate in a culture flask.
  5. Continue the further culture procedures according to standard protocols.

Application

Cell type Description
293 Sheared human Ad5 DNA-transformed cell line.
293T Human cell line expressing SV40 large T antigen
32D Murine myeloid cell
3LL Murine Lewis lung cancer cell
A10 Thoracic aortic smooth muscle of an embryonic rat
A2781 Glioblastoma cell
Ac2F Rat liver cell
AtT20 Murine anterior pituitary cell
Ba/F3 Murine Pro-B cell
BHK-21 Ba Hamster renal fibroblast cell
C2C12 Mouse myoblast cell
C3H10T1/2 Murine embryonic fibroblast cell
Caco-2 Human colonic adenocarcinoma cell
CHL/1U Neonatal Chinese Hamster Lung cell
CHO Chinese Hamster Ovary cell
CHO-K1 Chinese Hamster Ovary cell
Colo203 Human colonic adenocarcinoma cell
COS1 African green monkey kidney cell
COS7 African green monkey kidney cell
CTLL-2 Murine T-cell
DLP-1 Human colonic adenocarcinoma cell
DT40 Chicken B lymphocyte cell
DU145 Human prostate carcinoma cell
EBV transformed B cell
EJ-1 Human bladder carcinoma
ELM-D Murine erythroblastic leukemia cell
HeLa cell Human Uterine Cervical Carcinoma Cell
Hep3B Human hepatoma cell
Hepal-6 Murine hepatoma cell
Hepatocyte
HepG2 Hepatocellular carcinoma cell
HL-60 Human promyelocytic leukemia cell
HT-2 Murine cell
Huh-7 Human hepatocarcinoma cell
Jurkat Human T-cell line
K-562 Human Caucasian Chronic myelogenous leukaemia cell
Kato-III Human stomach carcinoma
Keratinocyte
KM12-LX Human Caucasian Chronic myelogenous leukaemia cell
L5178Y Murine lymphoma cell
L929 Murine fibroblast cell
LM Murine fibroblast cell
LNCap Human prostate adenocarcinoma cell
Lymphocyte
MCF-7 Human metastatic mammary carcinoma cell
MDCK Madin-Dar canine kidney cell
Microvascular Endothelial Cell
Molt-4 Human T-cell
NB-1/GOTO Human neuroblastoma
NCI-H441 Human lung adenocarcinoma epithelial cell
NIH3T3 Mouse embryonic fibroblast cell
P388 Murine leukimia cell
P3/x63-Ag8.U1 Murine myeloma cell
PC12 Rat pheochromocytoma cell

Order information

Package: 100ml/bottle, 20ml/bottle 

Document

SDS : CELLBANKER® 2

SDS : CELLBANKER® 2

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