Lipopolysaccharide (LPS) is a bacterial endotoxin and a major constituent of the cell walls of gram negative bacteria. Recombinant protein from E.coli usually contains high level of endotoxins. The removal of these endotoxins is highly necessary for downstream processes.
EtEraser SE Endotoxin Removal kit are designed to remove endotoxin contamination from aqueous solutions. The kits contain endotoxin removal resin binds to reduce endotoxin levels in protein samples by ≥ 99 % in less than 2 hours.
The kit includes a pre-packed endotoxin removal column 1.5 ml, Equilibration Buffer, Regeneration Buffer and pyrogen-free collection tube and tips. The column has a high binding capacity of > 2,000,000 EU / ml. This product may be reused up to five times if properly regenerated. The affinity resin is available in slurry and could be up-scalingin biopharmaceutical process.
• high stability — does not affect the activity of most of the biological sample
• high bonding capacity — >2000000 EU/ml
• non-toxic —–> Non-toxic food grade ligands, no cytotoxic effects poly lysine
• high protein recovery — >95% protein recovery for protein samples
• high removal efficiency — remove >99% endotoxin, endotoxin level in the sample can be less than 0.1 EU/ml after endotoxin removing process
• wide application range — can be used in endotoxin removal for proteins, peptides, antibodies, vaccines, polysaccharide and other biological samples
Catalog Number | Description | Kit Contents |
ER0015SE | EtEraserTM SE non-toxic endotoxin removal kit |
EtEraserTM SE non-toxic endotoxin removal pre-packed column 1.5 ml Equilibration buffer Regeneration buffer Pyrogen - free collection tube Pyrogen - free tips |
ER0100SE | EtEraserTM SE non-toxic endotoxin removal resin |
EtEraserTM SE non-toxic endotoxin removal resin 10 ml Regeneration buffer Equilibration buffer |